1. What is Peptide Reconstitution?

Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent to achieve a desired concentration for experimental or therapeutic use.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( Mass \) — Weight of peptide in milligrams (mg)
• \( Volume \) — Volume of solvent in milliliters (ml)

Explanation: This simple calculation determines the concentration of your peptide solution after reconstitution.

3. Importance of Accurate Reconstitution

Details: Proper reconstitution is critical for achieving consistent experimental results and ensuring correct dosing in therapeutic applications.

4. Using the Calculator

Tips: Enter the mass of your peptide in mg and the volume of solvent you plan to use in ml. Both values must be greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: What solvents are typically used for peptide reconstitution?
A: Common solvents include sterile water, PBS, or acetic acid solutions depending on peptide solubility and intended use.

Q2: How should I store reconstituted peptides?
A: Most reconstituted peptides should be aliquoted and stored at -20°C or -80°C to prevent degradation.

Q3: What concentration should I use for my experiments?
A: Optimal concentration depends on your specific application - consult literature or protocols for your particular peptide.

Q4: How long do reconstituted peptides remain stable?
A: Stability varies by peptide but typically ranges from weeks to months when properly stored.

Q5: Should I vortex or sonicate my peptide during reconstitution?
A: Gentle vortexing may help, but avoid excessive agitation or heating which could damage the peptide.