1. What is Protein Molarity?

Protein molarity refers to the concentration of a protein solution expressed in moles per liter (M). It's a fundamental measurement in biochemistry that allows researchers to compare protein concentrations on a molecular basis rather than just by mass.

2. How Does the Calculator Work?

The calculator uses the protein molarity formula:

Where:

• \( Concentration \) — Protein concentration in milligrams per milliliter (mg/mL)
• \( Molecular Weight \) — Protein molecular weight in kilodaltons (kDa)
• \( 1000 \) — Conversion factor to get micromolar (µM) units

Explanation: The formula converts mass concentration to molar concentration by accounting for the protein's molecular weight.

3. Importance of Protein Molarity

Details: Knowing the molar concentration is essential for experiments requiring precise stoichiometric ratios, enzyme kinetics studies, and when comparing activities of different proteins.

4. Using the Calculator

Tips: Enter protein concentration in mg/mL and molecular weight in kDa. Both values must be positive numbers. The result will be in micromolar (µM) units.

5. Frequently Asked Questions (FAQ)

Q1: Why use molarity instead of mg/mL?
A: Molarity accounts for molecular weight, allowing comparison of molecules on a per-particle basis rather than by mass.

Q2: How do I convert kDa to g/mol?
A: 1 kDa = 1000 g/mol, as 1 dalton is defined as 1 g/mol.

Q3: What if my protein is a multimer?
A: Use the molecular weight of the entire complex (sum of all subunits if homomultimer).

Q4: How accurate is this calculation?
A: It's mathematically precise, but accuracy depends on your concentration and molecular weight measurements.

Q5: Can I use this for nucleic acids?
A: Yes, the same formula works for any biomolecule when you know its concentration and molecular weight.