Tocris Molarity Formula:
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Molarity (M) is a measure of concentration, defined as the number of moles of solute per liter of solution. For Tocris compounds, accurate molarity calculation is essential for preparing solutions with precise concentrations for research purposes.
The calculator uses the molarity equation:
Where:
Explanation: The formula converts mass (mg) to moles using molecular weight, then divides by volume to get concentration.
Details: Accurate molarity is crucial for experimental reproducibility, proper dosing in cell culture, and ensuring consistent results in biochemical assays.
Tips: Enter mass in mg, molecular weight in g/mol, and volume in mL. All values must be positive numbers.
Q1: Why is molecular weight important?
A: Molecular weight converts mass to moles, which is necessary for molarity calculations as it's a mole-based concentration unit.
Q2: What are typical molarity ranges?
A: Stock solutions are often in mM (millimolar) range (1-100 mM), while working concentrations are often in µM or nM range.
Q3: Should I account for purity?
A: Yes, if your compound isn't 100% pure, adjust the mass input accordingly (e.g., for 95% purity, multiply mass by 0.95).
Q4: How does temperature affect molarity?
A: Molarity is temperature-dependent as volume changes with temperature. For precise work, measure at the temperature you'll be using the solution.
Q5: Can I use this for any compound?
A: Yes, this works for any compound as long as you know its molecular weight. The calculator is particularly useful for Tocris compounds.