1. What is Oligo Reconstitution?

Oligo reconstitution is the process of dissolving oligonucleotides (short DNA or RNA molecules) in a suitable buffer to achieve a desired concentration for experimental use.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( Amount \) — Amount of oligonucleotide in nanomoles (nmol)
• \( Concentration \) — Desired concentration in micromolar (μM)
• \( Volume \) — Volume of solvent needed in microliters (μL)

Explanation: The formula calculates the volume of liquid needed to dissolve your oligonucleotide to achieve a specific concentration.

3. Importance of Proper Reconstitution

Details: Accurate reconstitution is crucial for experimental consistency, ensuring proper molar ratios in reactions, and achieving reproducible results.

4. Using the Calculator

Tips: Enter the amount of oligo in nmol and desired concentration in μM. Typical working concentrations range from 10-100 μM for most applications.

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for reconstitution?
A: TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) or nuclease-free water are most commonly used.

Q2: How should I store my reconstituted oligo?
A: Store at -20°C for long-term storage. Avoid repeated freeze-thaw cycles by aliquoting.

Q3: How do I measure oligo amount?
A: The amount is typically provided by the manufacturer (in nmol) based on synthesis scale and purification.

Q4: What if my oligo doesn't dissolve completely?
A: Try heating at 55°C for 1-2 minutes or vortexing. Some sequences may require more vigorous mixing.

Q5: How accurate is this calculation?
A: The calculation is mathematically precise, but actual concentration depends on accurate measurement of both the oligo amount and final volume.