1. What is the IDT Resuspension Formula?

The IDT (Integrated DNA Technologies) resuspension formula calculates the volume of buffer needed to resuspend an oligonucleotide to achieve a desired concentration. This is essential for molecular biology experiments.

2. How Does the Calculator Work?

The calculator uses the IDT resuspension formula:

Where:

• \( V \) — Volume of buffer needed (μL)
• \( nmol \) — Amount of oligonucleotide (nmol)
• \( desired\_μM \) — Target concentration (μM)

Explanation: The formula accounts for the molecular weight and desired concentration to determine the appropriate resuspension volume.

3. Importance of Proper Resuspension

Details: Accurate resuspension is crucial for consistent experimental results, proper primer working concentrations, and reproducible PCR reactions.

4. Using the Calculator

Tips: Enter the amount of oligo in nmol (as provided by IDT) and your desired concentration in μM. The calculator will determine the volume of buffer needed.

5. Frequently Asked Questions (FAQ)

Q1: What buffer should I use for resuspension?
A: IDT recommends using TE buffer (10 mM Tris, 0.1 mM EDTA, pH 8.0) or nuclease-free water.

Q2: What is a typical working concentration for primers?
A: Most PCR reactions use primers at 0.1-1 μM final concentration.

Q3: How should I store resuspended oligos?
A: Store at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.

Q4: What if my oligo doesn't dissolve completely?
A: Heat at 55°C for 1-2 minutes and vortex. Some modifications may require longer heating.

Q5: Can I use this for modified oligos?
A: Yes, but some modifications may affect solubility. Check IDT's recommendations for specific modifications.