Resuspension Volume Calculator

Resuspension Volume Equation:

\[ V = \frac{nmol}{concentration_{\mu M}} \times 1000 \]

Resuspension Volume:

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1. What is Resuspension Volume?

Resuspension volume is the amount of liquid needed to dissolve a dry compound to achieve a desired concentration. It's commonly used in molecular biology and chemistry to prepare stock solutions from powdered reagents.

2. How Does the Calculator Work?

The calculator uses the resuspension volume equation:

\[ V = \frac{nmol}{concentration_{\mu M}} \times 1000 \]

Where:

\( V \) — Resuspension volume (μL)
\( nmol \) — Amount of substance in nanomoles
\( concentration_{\mu M} \) — Desired concentration in micromolar

Explanation: The equation converts between nmol and μM while adjusting for the final volume unit (μL).

3. Importance of Resuspension Volume

Details: Accurate resuspension is crucial for experimental reproducibility, proper reagent storage, and ensuring correct concentrations for downstream applications.

4. Using the Calculator

Tips: Enter the amount of substance in nmol and desired concentration in μM. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why multiply by 1000 in the equation?
A: The multiplication converts liters to microliters (1 L = 1000 mL = 1,000,000 μL) since we're working with μL as the final volume unit.

Q2: What's the difference between nM and μM?
A: 1 μM = 1000 nM. Micromolar (μM) is 1000 times more concentrated than nanomolar (nM).

Q3: Can I use this for proteins and DNA?
A: Yes, this calculation works for any substance where you know the amount in nmol and want a specific μM concentration.

Q4: How do I measure nmol of a substance?
A: Use the molecular weight and mass. nmol = (mass in μg) × 1000 / molecular weight.

Q5: What if my desired concentration is in mM?
A: Convert mM to μM first (1 mM = 1000 μM) before using the calculator.